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Mothers were asked to complete the Quantitative Checklist for Autism in Toddlers (Q-CHAT), a measure of autistic traits in children 18 to 24 months old.

f T (but not p T) levels were positively associated with scores on the Q-CHAT.

f E and p T levels showed no sex differences and no relationships with f T levels.

f T levels were the only variable that predicted Q-CHAT scores.

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Fetal testosterone (f T) and fetal estradiol (f E) levels were measured in amniotic fluid from pregnant women (n = 35) following routine second-trimester amniocentesis.

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